To develop a rapid detection test strip based on specific antigen-antibody reactions, which can be used for rapid and highly sensitive detection of camel milk adulteration.

By utilizing the structural differences of α-lactalbumin between cow’s milk and camel milk, specific antibodies were developed. Then, an indirect competitive colloidal gold immunochromatographic technique was adopted to develop the detection test strip.

The test strip could detect adulteration in camel milk, with a sensitivity of up to 0.03%. In addition, it could also be used to detect adulteration in camel milk powder and its detection sensitivity could reach 0.1% when calculated based on milk powder.

The colloidal gold rapid chromatographic detection test strip developed in this study has high sensitivity, convenient operation and low cost. It greatly improves the detection efficiency and plays an important role in the rapid detection and screening of camel milk adulteration.