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Outline
To explore the efficient extraction method and biological activity of black Crabapple polysaccharides (BCP).
Using black Crabapple as the raw material, the extraction conditions of BCP were optimized through single-factor experiments combined with response surface methodology. The monosaccharide composition was analyzed by high performance liquid chromatography, and its in vitro antioxidant activity was evaluated based on the scavenging rates of 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) radicals,2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonate acid) diammonium salt cation (ABTS⁺) radicals and hydroxyl radicals.
The optimal extraction process for BCP was a liquid-to-solid ratio of 20:1 (mL:g), extraction temperature of 72 ℃ and extraction time of 45 min, yielding a polysaccharide recovery rate of (19.98±0.25)%. BCP consisted of 8 kinds of monosaccharides, including glucose, galactose and arabinose, confirming its identity as an acidic heteropolysaccharide. Antioxidant experiments demonstrated that BCP exhibited concentration-dependent scavenging capabilities against DPPH radicals, ABTS⁺ radicals and hydroxyl radicals. At a mass concentration of 1.0 mg/mL, the inhibition rates for these 3 kinds of radicals were (84.70±0.38)%, (71.20±0.91)% and (92.40±0.51)% respectively, indicating its favorable in vitro antioxidant activity.
This study optimizes the extraction process of BCP and proves its excellent antioxidant properties, providing a theoretical reference for promoting the high-value development of black Crabapple.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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