Full
Outline
To establish a quantum dot microsphere based fluorescent immunochromatographic method for the rapid, sensitive and simultaneous detection of clothianidin and thiamethoxam in ginger.
In this study, the labeling pH, antibody labeling concentration and reaction buffer system of the 2 items were optimized.The fluorescence signal intensity of the test line and quality control line on the test strip was measured by the quantum dot microspheres test strip detector. The concentration of clothianidin and thiamethoxam was used as the abscissa, and the ratio of fluorescence signal intensity of the test line and quality control line was used as the ordinate to establish a standard curve.
The quantitative detection ranges were 0.0625–8.0000 μg/L for clothianidin and 0.125–16.000 μg/L for thiamethoxam. The minimum limits of detection for thiamethoxam were 0.032 μg/L and for thiamethoxam were 0.070 μg/L.The recovery rates of standard addition for clothianidin ranged from 84.60% to 124.05%, and for thiamethoxam from 88.27% to 116.40%. The cross-reaction rate with 8 kinds of common drugs is less than 1%.
This method features unified sample pretreatment, simple and rapid operation, high sensitivity and simultaneous quantitative results for 2 analytes, making it suitable for on-site screening of large batches of samples.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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