Full
Outline
To establish a method for the rapid determination of 20 kinds of hormone drugs in pork by ultra performance liquid chromatography coupled with quadrupole-orbitrap high resolution mass spectrometry.
The samples were extracted with water-acetonitrile solution and centrifuged to discard precipitation followed by a salt partition using sodium chloride and sodium sulphate. The acetonitrile extraction solution was purified via solid phase extraction using an HyperSep Retain-PEP column, then separated by an Thermo Hypersil GOLD C18 column (100 mm×2.1 mm, 1.9 μm), ionized in positive ion mode, and detected by ultra performance liquid chromatography coupled with quadrupole-orbitrap high resolution mass spectrometer with full mass/data dependent secondary acquisition (Top N) [Full MS/dd-MS2 (Top N)] mode.
The 20 kinds of hormone drugs showed good linear relationships in their respective ranges, with determination coefficients (r2)≥0.9902. The average recoveries were 36.56%-119.43% with the relative standard deviations between 0.9% and 9.8%. The limits of detection were in the ranges of 0.1–0.5 μg/kg and the limits of quantitation were at 1.0 μg/kg.
This method demonstrates good accuracy and stability, making it suitable for the detection of 20 kinds of hormone drugs in pork.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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