Full
Outline
To investigate the microencapsulation efficiency and stability enhancement effect of lysozyme and gelatin composite wall materials on anthocyanins from Lycium ruthenicum Murray, and optimize the process parameters for expanding the application of anthocyanins in food and health supplements.
Anthocyanin microcapsules were prepared by spray drying using lactoferrin and gelatin as composite wall materials. The effects of wall material volume ratio, core-wall ratio, pH, embedding temperature and inlet air temperature on the anthocyanin content of the microcapsules were investigated through single-factor experiments, followed by process parameter optimization via orthogonal experiments; the structure of the microcapsules was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy and X-ray diffraction analysis, and their storage stability under different temperature and light conditions was tested.
The order of factors influencing microencapsulation was identified as pH>core-to-wall ratio>wall material volume ratio>inlet air temperature>embedding temperature, with the optimal process combination being A3B4C2D1E3 (wall material volume ratio 8:1, core-to-wall ratio 4:1, pH=2, embedding temperature 40 ℃, inlet air temperature 180 ℃). Under these conditions, the anthocyanin content in the microcapsules reached 152.83 mg. Structural characterization revealed that the microcapsules exhibited a spherical aggregated morphology, and the composite wall material did not disrupt the chemical structure of anthocyanins while forming a more stable semi-crystalline structure. Stability tests demonstrated that under storage conditions of 4 ℃ and 37 ℃, the retention rate of microencapsulated anthocyanins was significantly higher than that of the unencapsulated group. After 16 days of light exposure, the remaining amount in the microencapsulated group was markedly greater, indicating significantly improved thermal and light stability.
The microencapsulation technology using lysozyme and gelatin composite wall materials can efficiently encapsulate anthocyanins from Lycium ruthenicum Murray and enhance their stability, and the optimized process parameters provide key technical support for the industrial application of anthocyanins from Lycium ruthenicum Murray.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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