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Determination of 22 kinds of phytochemicals in Ficus carica tea by high performance liquid chromatography
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Rong-Kang ZHAI1, 2, Shang LI3, Feng-Jia ZHENG4, Jun WANG2, Xue-Song ZHANG2, Ying LU2, Jing-Ming YANG2, Wei-Sheng XU2, 5, 6, *, Fu-Yun ZHANG1, *
Journal of Food Safety & Quality | 2026, 17(6) : 287 - 294
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Journal of Food Safety & Quality | 2026, 17(6): 287-294
• Food Analysis and Detection •
Determination of 22 kinds of phytochemicals in Ficus carica tea by high performance liquid chromatography
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Rong-Kang ZHAI1, 2, Shang LI3, Feng-Jia ZHENG4, Jun WANG2, Xue-Song ZHANG2, Ying LU2, Jing-Ming YANG2, Wei-Sheng XU2, 5, 6, *, Fu-Yun ZHANG1, *
Affiliations
  • 1.College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China
  • 2.National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China
  • 3.Chongqing Jiulongpo District Center for Disease Control and Prevention, Chonging 400039, China
  • 4.Shandong Center for Disease Control and Prevention, Ji’nan 250000, China
  • 5.Key Laboratory of Public Nutrition and Health, National Institute for Nutrition and Health, Beijing 100050, China
  • 6.Key Laboratory of Nutrition, Jiangxi Provincial Health Commission, Nanchang 330006, China
doi: 10.19812/j.cnki.jfsq11-5956/ts.20251016001
Outline
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Objective

To establish a method for determation of 22 kinds of phytochemicals in Ficus carica by high performance liquid chromatography.

Methods

Ficus carica samples were ground, then mixed with distilled water and sonicated at 50 ℃ for 30 min before centrifugation. Ficus carica tea samples were soaked in 100 ℃distilled water. After soaking, samples were separated using gradient elution with 0.15% phosphoric acid solution-acetonitrile as mobile phase on an Agilent Eclipse XDB C18 (4.6 mm×100 mm, 3.5 μm) column. Column temperature was maintained at 30 ℃ with a 10 μL injection volume. Plant chemical content was determined using the external standard method with wavelength switching technology.

Results

The 22 kinds of phytochemicals were detected within 50 min with baseline separation achieved, and the linear correlation coefficients (r2) were all greater than 0.9995. The limits of detection ranged from 0.06 to 0.35 μg/g, the limits of quantitation ranged from 0.20 to 1.18 μg/g, and the spiked recovery rates ranged from 84.7% to 106.6%, relative standard deviations were 1.3%–9.5%.

Conclusion

The method is sensitive, accurate and reproducible, and is suitable for determining the 22 kinds of phytochemicals in Ficus carica tea.

phytochemicals  /  Ficus carica tea  /  high performance liquid chromatography
翟容康, 李上, 郑凤家, 王珺, 张雪松, 陆颖, 杨晶明, 徐维盛, 张付云. 高效液相色谱法测定无花果茶中22种植物化学物含量. 食品安全质量检测学报, 2026 , 17 (6) : 287 -294 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20251016001
Rong-Kang ZHAI, Shang LI, Feng-Jia ZHENG, Jun WANG, Xue-Song ZHANG, Ying LU, Jing-Ming YANG, Wei-Sheng XU, Fu-Yun ZHANG. Determination of 22 kinds of phytochemicals in Ficus carica tea by high performance liquid chromatography[J]. Journal of Food Safety & Quality, 2026 , 17 (6) : 287 -294 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20251016001
Appendix
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Year 2026 volume 17 Issue 6
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20251016001
  • Receive Date：2025-10-16
  • Online Date：2026-11-05
Article Data
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History
  • Received：2025-10-16
Funding
Affiliations
    1.College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China
    2.National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China
    3.Chongqing Jiulongpo District Center for Disease Control and Prevention, Chonging 400039, China
    4.Shandong Center for Disease Control and Prevention, Ji’nan 250000, China
    5.Key Laboratory of Public Nutrition and Health, National Institute for Nutrition and Health, Beijing 100050, China
    6.Key Laboratory of Nutrition, Jiangxi Provincial Health Commission, Nanchang 330006, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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