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Rapid identification of the authenticity of Mentha by real-time fluorescence quantitative polymerase chain reaction
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Yu-Xiu DENG1, 2, Rui LI1, 2, Hai-Bo WANG1, 2, Jian WU1, 2, Xiao-Bin CAO3, Huang ZHANG4, Hao-Ting CHEN4, Shang-Chao YANG1, 2, *
Journal of Food Safety & Quality | 2026, 17(5) : 51 - 58
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Journal of Food Safety & Quality | 2026, 17(5): 51-58
• Special Topic: Food Authentication and Traceability •
Rapid identification of the authenticity of Mentha by real-time fluorescence quantitative polymerase chain reaction
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Yu-Xiu DENG1, 2, Rui LI1, 2, Hai-Bo WANG1, 2, Jian WU1, 2, Xiao-Bin CAO3, Huang ZHANG4, Hao-Ting CHEN4, Shang-Chao YANG1, 2, *
Affiliations
  • 1.Guangxi—asean Food Inspection and Testing Center, Nanning 537100, China
  • 2.Technology Innovation Center of Natural Flavors and Fragrances, State Administration for Market Regulation, Nanning 537100, China
  • 3.Jiangxi Kangyi Food Co., Ltd., Yichun 336000, China
  • 4.Guangzhou Double Helix Gene Technology Co., Ltd., Guangzhou 510000, China
doi: 10.19812/j.cnki.jfsq11-5956/ts.20251014003
Outline
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Objective

To establish a rapid method for identifying the authenticity of medicinal and edible plant Mentha authenticity by real-time fluorescence quantitative polymerase chain reaction.

Methods

Based on TaqMan probe technology (TaqMan), upstream and downstream primers, and a TaqMan probe were designed targeting the conserved region within the intron of the chloroplast DNA-encoded ribosomal protein L16 (rpl16) gene to specifically recognize 3 independent regions on the target sequence. A TaqMan reaction system was established to achieve rapid and accurate identification of Mentha and its homologous species.

Results

The method demonstrated good specificity showing amplification exclusively in Mentha samples, and no amplification was observed in common adulterants. In terms of sensitivity, the cycle threshold (Ct) values exhibited a linear relationship with the logarithm of template concentration over a broad range (0.01–10000.00 fg/μL) (r2=0.9999). The absolute limit of detection for Mentha plasmid DNA was 0.01 fg/μL, and the limit of detection for Mentha in real samples reached 0.1% (quality score). Stability testing results showed that all negative controls remained stable without non-specific amplification.

Conclusion

This method exhibits high specificity, sensitivity and stability, enabling effective differentiation between Mentha and its adulterants.

real-time fluorescent polymerase chain reaction  /  Mentha  /  authenticity verification  /  rapid detection
邓玉秀, 李锐, 王海波, 巫坚, 曹孝斌, 张璜, 陈灏挺, 杨尚超. 实时荧光定量聚合酶链式反应法快速鉴别薄荷真实性. 食品安全质量检测学报, 2026 , 17 (5) : 51 -58 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20251014003
Yu-Xiu DENG, Rui LI, Hai-Bo WANG, Jian WU, Xiao-Bin CAO, Huang ZHANG, Hao-Ting CHEN, Shang-Chao YANG. Rapid identification of the authenticity of Mentha by real-time fluorescence quantitative polymerase chain reaction[J]. Journal of Food Safety & Quality, 2026 , 17 (5) : 51 -58 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20251014003
Appendix
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Year 2026 volume 17 Issue 5
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20251014003
  • Receive Date：2025-10-14
  • Online Date：2026-11-05
Article Data
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History
  • Received：2025-10-14
Funding
Affiliations
    1.Guangxi—asean Food Inspection and Testing Center, Nanning 537100, China
    2.Technology Innovation Center of Natural Flavors and Fragrances, State Administration for Market Regulation, Nanning 537100, China
    3.Jiangxi Kangyi Food Co., Ltd., Yichun 336000, China
    4.Guangzhou Double Helix Gene Technology Co., Ltd., Guangzhou 510000, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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