Full
Outline
To establish an immunochromatographic technique that can provide both visual and fluorescent signal readout at the same time. To evaluate the practical application performance of this technology for high-sensitivity and high-accuracy detection of trace tadalafil in complex matrices of energy drinks.
In this study, magnetic nanoparticles were first combined with quantum dots to prepare magnetic quantum dot composite materials, which were then used to label the anti-tadalafil antibody, thereby constructing a highly sensitive and specific magnetic quantum dot probe. Meanwhile, a pre-incubation step was introduced to effectively eliminate matrix interference. During the experimental process, key conditions including the antibody labeling amount, type of blocking agent, resuspension buffer system and composition of the incubation solution were systematically optimized.
Experimental data showed that the dual-modal immunochromatographic technology achieved a visual limit of detection for tadalafil of 0.5 ng/mL in the colorimetric mode and 1 ng/mL in the fluorescent mode.Additionally, the technology exhibited excellent specificity with no cross-reactivity observed with structural analogs such as sildenafil and vardenafil.
The dual-mode immunochromatographic technique based onmagnetic quantum dots ingeniously combine the high purification capability of magnetic separation technology with the high fluorescence sensitivity of quantum dots, offering significant advantages such as simplicity of operation and strong anti-interference ability. This technique can achieve rapid screening and preliminary quantification of tadalafil in functional foods on-site, providing a practical and efficient technical solution for the detection of illegal additives in functional foods.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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