Full
Outline
To establish a rapid detection method suitable for the determination of chlorpyrifos residues in vegetables based on the colloidal gold immunochromatographic technology.
The pretreatment conditions such as extraction solvent, extraction time and detection temperature were systematically optimized to determine the optimal rapid detection parameters. Additionally, the performance indicators of this rapid detection method, including false negative rate, false positive rate and relative accuracy, were confirmed.
Using 8.0 mL of 0.1 mol/L phosphate buffer saline solution as the sample extraction solvent, setting the extraction time to 3 min, and controlling the detection temperature within the range of 15–35 ℃ were determined as the optimal parameters for rapid detection. The experimental results showed that the false negative rate and false positive rate of this method were both 0%, with a relative accuracy of 100%; the rapid detection results of 20 vegetable samples were consistent with those obtained by the liquid chromatography-tandem mass spectrometry method.
This method demonstrates excellent detection performance and is suitable for on-site rapid detection and large-scale screening of chlorpyrifos residues in vegetables.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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