Full
Outline
To establish a rapid determination method for 11 kinds of yellow industrial dyes including auramine O and so on in Durio zibetuinus Murr by high performance liquid chromatography-tandem mass spectrometry.
Durio zibetuinus Murr pulp samples were extracted by ultrasound with 80% acetonitrile aqueous solution, Durio zibetuinus Murr peel samples were extracted by ultrasound with acetonitrile, purified with 60 mg ethylenediamine-N-propyl silanized silica gel. The analytes were separated by Agilent Poroshell 120 EC-C18(3.0 mm×100 mm, 2.7 μm) chromatographic column with methanol-0.1% formic as mobile phase by gradient elution.The determination was carrried out by high performance liquid chromatography-tandem mass spectrometry in electrospray positive ion scanning and multi-reaction monitoring mode, and quantified by external standard matrix standard curve method.
The linear correlation coefficients (r) of 11 kinds of industrial dyes were 0.99346–0.99996 within the range of 0.2–10.0 ng/mL, indicating a good linear relationship. The limits of detection were 1.0 μg/kg, and the limits of quantitation were 2.0 μg/kg. The recoveries of 11 kinds of industrial dyes ranged from 74.6% to 99.3% and the relative standard deviations were 1.1% to 14.3% at 6 kinds of parallel spiked tests with limits of quantitation of 1, 3 and 10 times for Durio zibetuinus Murr substrate.
This method is easy to operate, accurate in results, and suitable for rapid screening and quantitative analysis of 11 kinds of yellow industrial dyes in Durio zibetuinus Murr, providing technical support for food safety supervision.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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