Full
Outline
To establish a method to detect fluoride content based on the fluoride riboswitch transcription system and clustered regularly interspaced short palindromic repeats-associated protein 12a(CRISPR/Cas12a).
The system utilized fluoride riboswitch to regulate transcription of full-length or truncated RNA in the presence/absence of fluoride, which was then detected by CRISPR/Cas12a-mediated fluorescence signal. Selectivity and interference analyses were conducted to optimize CRISPR/Cas12a detection system, and the method was applied to measure fluoride content in Euphausia superba.
This study successfully established a fluorescence detection method based on a fluoride riboswitch transcription reaction system and CRISPR/Cas12a, which completed sample detection within 1.5 h with the results consistent with those obtained by the national standard fluoride ion-selective electrode method.
The fluoride detection method established in this study for Euphausia superba based on a fluoride riboswitch transcription reaction system and CRISPR/Cas12a, demonstrates high specificity and accuracy, showing potential for fluoride content analysis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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