To develop a method for the simultaneous determination of 30 kinds of pesticide and veterinary drug residues across 10 classes-including sulfonamides, tetracyclines, quinolones, macrolides, nitroimidazoles, triphenylmethanes, β-lactams, hormones, organophosphorus compounds and carbamates in aquatic products by automated solid-phase extraction coupled with ultra performance liquid chromatography-tandem mass spectrometry.

Samples were extracted with acetonitrile containing formic acid, purified using HLB solid-phase extraction cartridges, and separated on a Waters ACQUITY UPLC BEH C₁₈ column with a mobile phase consisting of 0.1% formic acid in acetonitrile and 0.1% formic acid in water, and analyzed by a SCIEX\Triple Quad 5500+ tandem mass spectrometer.

The 30 kinds of compounds exhibited good linearity within the concentration range of 5–100 μg/L, with correlation coefficients ranging from 0.9914 to 0.9998. The limits of detection were less than or equal to 0.025 mg/kg, and the limits of quantitation were less than or equal to 0.050 mg/kg. Mean recoveries for spiked samples ranged from 62.8% to 108.1%, with relative standard deviations of 1.0%–14.7% (n=6).

The method demonstrates high precision, good accuracy and a high degree of automation, making it suitable for high-throughput screening of pesticide and veterinary drug residues in aquatic products.