Full
Outline
To investigate the conditions for inducing expression levels of α-L-rhamnose glycosidase RhaB2 and its conversion of rutin, thereby enhancing the conversion rate of rutin to isoquercitrin.
Single-factor experiments examined the effects of induction temperature, duration and inducer concentration on enzyme expression levels. The effects of pH, temperature, incubation time and enzyme loading on RhaB2-mediated rutin conversion were also investigated.
Optimal expression conditions for recombinant α-L-rhamnose glycosidase RhaB2 in Escherichia coli BL21(DE3) were: Induction temperature 28 °C;induction time 20 hours; IPTG concentration 0.1 mmol/L. The optimal temperature for rutin conversion was 50 °C,with an optimal pH of 5.0. Under these conditions, a 2-hour reaction at a 2:1 enzyme-to-substrate mass ratio achieved a conversion rate of 99.96%.
In this study, a highly efficient heterologous expression system for recombinant α-L-rhamnosidase RhaB2 is successfully constructed. Under the technological conditions where the mass ratio of the enzyme to the substrate is 2:1 and the reaction time is 2 hours, the conversion rate of rutin can reach 99.96%, thus enabling the highly efficient and targeted preparation of isorhamnetin.
7
1
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
