Full
Outline
To establish a method for the determination of 9 kinds of stevioside compounds in aquatic products by ultra performance liquid chromatography-tandem mass spectrometry with isotope internal standard.
The sample was extracted with methanol aqueous solution (1:1, V:V), separated on a CSH C18 chromatographic column, and eluted with a gradient. The sample was analyzed in multiple reaction monitoring mode using electrospray negative ionization mode and quantified by internal standard method.
The 9 kinds of stevioside compounds showed good linear relationships in the range of 0.1–100.0 ng/mL. The limits of detection were 2.0 µg/kg, and the limits of quantitation were 5.0 µg/kg. The spiked recoveries of the method at low, medium and high spiked levels showed that the recoveries were in the range of 90.16%–103.69% with the relative standard deviations of 2.24%–6.38%. The method was used to analyze aquatic products, with a detection rate of 12%.
The method is simple, efficient, and highly accurate and sensitive, making it suitable for the determination of 9 kinds of stevioside compounds in aquatic products.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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