Full
Outline
To establish a method for the determination of 7 kinds of active components in the characteristic Dendrobium officinale and Dendrobium devonianum in Yunnan Province by deep eutectic solvents(DESs)-methanol-water extraction and ultra performance liquid chromatography-tandem mass spectrometry, and conduct identification research on the characteristic Dendrobium officinale and Dendrobium devonianum in Yunnan Province.
After ultrasonic extraction of the samples in a DESs-methanol-water solution ice bath, ultra performance liquid chromatography-tandem mass spectrometry was employed for analysis in multiple reaction monitoring mode, with chemometric data analysis conducted.
The correlation coefficients for the 7 kinds of active ingredients within a certain mass concentration range were relatively good. The limits of detection and limits of quantitation ranged from 0.006 mg/kg to 0.030 mg/kg and 0.020 mg/kg to 0.100 mg/kg, respectively. The relative standard deviations ranged for precision, repeatability and stability were 0.95% to 5.45%. Compared to Dendrobium officinale, Dendrobium devonianum exhibited higher levels of ginsenoside, isoquercitrin, apigenin and protocatechuic acid, while showing lower levels of ginsenoside; the content of naringin and vanillic acid remained relatively consistent between the 2 kinds of species.
The sensitivity and stability of the method are relatively good. Dendrobium officinale and Dendrobium devonianum can be effectively discriminated by orthogonal partial least squares discriminant analysis. The research results can provide a reference for the quality evaluation of the characteristic dendrobium in Yunnan Province.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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