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Outline
To investigate the inhibitory effect of purple sweet potato anthocyanins (PSPA) on oxidative stress, and elucidate its underlying molecular mechanism.
The potential targets of PSPA active components and oxidative stress were screened through public databases to obtain overlapping targets, which subsequently underwent gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) enrichment analyses to preliminarily elucidate the underlying mechanisms. An oxidative stress model was established in BV2 microglial cells using lipopolysaccharide (LPS) induction. Cells were pretreated with low or high doses of PSPA. The levels of nitric oxide (NO), malondialdehyde (MDA), glutathione (GSH), and the activity of catalase (CAT), superoxide dismutase (SOD) were measured using enzyme-linked immunosorbent assay. Apoptosis was assessed via Hoechst staining and flow cytometry, and related protein expression was analyzed by Western blot.
The main active components of PSPA were identified as peonidin and cyanidin. Network pharmacology analysis identified 253 PSPA-related targets and 1178 oxidative stress-related targets, with 86 overlapping targets. Topological analysis suggested that PSPA might counteract oxidative stress by targeting key proteins such as protein kinase B alpha(AKT1), epidermal growth factor receptor (EGFR) and heat shock protein 90 alpha family class A member 1(HSP90AA1). Molecular docking confirmed strong binding affinity between PSPA and the top 3 core targets. GO and KEGG analyses indicated that PSPA was involved in regulating cellular components such as the cytosol and cytoplasm; biological processes including activation of the phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) signaling pathway and protein phosphorylation; and pathways related to chemical carcinogenesis–reactive oxygen species. Cellular experiments demonstrated that PSPA significantly reduced NO and MDA levels, increased GSH content and CAT and SOD activity, inhibited abnormal apoptosis, and modulated PI3K/AKT phosphorylation.
PSPA effectively alleviates LPS-induced oxidative stress damage in BV2 microglial cells, likely through direct activation of the PI3K/AKT signaling pathway.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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