Full
Outline
To develop an intelligent, highly sensitive rapid detection platform for enrofloxacin (ENR) to address the issues of high cost and lengthy processing times associated with traditional detection methods, as well as the reliance on manual operation and insufficient identification accuracy of existing digital immunoassay technologies.
Based on automated imaging equipment and deep learning algorithms, a digital immunoassay system using polystyrene microspheres as signal carriers was constructed, combining immunoreaction and magnetic separation technologies to achieve quantitative detection. The system integrated a high-precision automated microdisplacement platform, adopted the Faster-RCNN algorithm to achieve rapid recognition of microspheres, and introduced the NAFNet image enhancement algorithm to improve the imaging quality.
The platform was capable of achieving high sensitivity detection of ENR (limit of detection 19.35 pg/ml, linear range 0.5–100.0 ng/mL), with test recoveries ranging from 104%–111%, and had made significant breakthroughs in detection efficiency, automation and anti-interference capability.
The study develops a deep learning-based automated immunoassay platform capable of stable image acquisition and accurate recognition of microspheres. The deep learning algorithm provides accurate and reliable counting, and the immunoassay system establishes features a wide linear range, low detection limit and high sensitivity. It performs well in the detection of milk samples and is suitable for the rapid automated detection of enrofloxacin in food.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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