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Outline
To establish a method for determination of 9 kinds of biogenic amines in aquatic products by tandem ion chromatography-ultraviolet detection (IC-UV).
Fish samples were ultrasonically extracted with 0.2 mmol/L methanesulfonic acid solution then protein was precipitated with acetonitrile, and analyzed after filtration through a membrane. The 9 kinds of biogenic amines and coexisting ions in the samples were separated on an IonPac CS17 cation exchange chromatographic column (250 mm×4 mm, 7 µm). The experimental conditions, including eluent condition, column temperature and detector wavelength range, were optimized to achieve optimal performance.
The 9 kinds of biogenic amines exhibited wide linear ranges: Octopamine, tyramine and tryptamine showed good linearity in the mass concentration range of 0.2–20.0 mg/L, putrescine, cadaverine, spermidine and spermine in the mass concentration range of 0.02–20.00 mg/L; and histamine and phenethylamine in the mass concentration range of 0.10–20.00 mg/L; with linear correlation coefficients (r2)≥0.9960 and satisfactory recoveries (83.8%–122.9%). The limits of detection for putrescine, cadaverine, histamine, phenethylamine, spermidine, spermine, octopamine, tyramine and tryptamine were 0.23, 0.30, 1.63, 1.56, 0.21, 0.26, 1.97, 2.50 and 2.88 mg/kg, respectively.
This method is simple, eliminates the complex derivatization pretreatment, and offers excellent selectivity and high sensitivity, making it suitable for the detection and analysis 9 kinds of biogenic amines in aquatic products.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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