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Outline
To investigate process factors affecting the stability of rapid testing methods for mycotoxins in Triticum aestivum L. and Zea mays L..
To used Triticum aestivum L. and Zea mays L. as primary grain samples, a systematic evaluation was conducted on: Screening rates (99%, 97%, 95%, 93%, 91%)mixing methods (manual, mechanical single/double mixing), sample sizes (300, 400, 500, 600, 700 g), and 3 kinds of commercially available rapid testers (SinoTech, FlyingTest, Charm) on detection results for aflatoxin B1, zearalenone and deoxynivalenol.
Toxin detection levels in raw grain correlated positively with sieving rate, reached a maximum of 667.61 μg/kg. However, variation among the 3 kinds of toxins differed significantly, likely due to their distinct distribution characteristics. Mechanical bidirectional mixed ensured thorough toxin dispersion, reduced detection error to 8.1%. Increasing sample mass enhanced result stability but complicated mixing procedures. Furthermore, all 3 kinds of rapid testing instruments (SinoTech, FlyingTest and Charm) exhibited measurement errors during toxin detection,potentially attributable to operational procedures and system calibration.
Optimising sample handling protocols effectively enhances the accuracy of rapid mycotoxin detection in grain, providing a theoretical basis for ensuring food safety and testing efficiency.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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