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Objective To obtain excellent lactic acid bacteria with bacteriostatic activity from fermented bamboo shoots and analyze their antibacterial substances. Methods Using Liuzhou traditional fermented bamboo shoots as raw materials, Staphylococcus aureus and Escherichia coli as indicator bacteria, and inhibition zone diameter as evaluation indicator, the Oxford Cup method was used to screen lactic acid bacteria with higher antibacterial properties. The strain was identified by morphological observation and molecular biological technique. Finally, the effects of pH, temperature and enzyme treatment on the antibacterial activity of the fermentation supernatant of the strain were analyzed. Results A total of 26 strains of lactic acid bacteria were isolated from Liuzhou traditional fermented bamboo shoots, among which 10 strains had antibacterial activity against Staphylococcus aureus and Escherichia coli, and strain LZ-6 had the strongest antibacterial activity, with a diameter of more than 15 mm for the inhibition zone. The strain LZ-6 was identified as Limosilactobacillus fermentum. The antibacterial substances produced by strain LZ-6 exhibit good antibacterial activity only under acidic conditions and had good thermal stability. After protease treatment, there was no significant change in antibacterial activity (P>0.05), indicated that the protein in the fermentation supernatant had no antibacterial effect. Conclusion A fermented lactobacillus LZ-6 with good antibacterial activity is screened from Liuzhou traditional fermented bamboo shoots. Its antibacterial substances are organic acids with stable activity and are not affected by temperature and enzymes. The lactic acid bacteria derived from fermented bamboo shoots obtained in this study can be used in the fields of sour bamboo shoot fermentation and biological preservatives, and lay the foundation for the separation, identification and mechanism research of antibacterial substances.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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