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Objective To establish a method for determination of 17 kinds of amino acids in 3 kinds of animal blood tofu products (duck, pig, sheep) by ultra performance liquid chromatography with pre-column derivatization. Methods The samples were hydrolyzed in 6 mol/L HCl for 24 h at 110 ℃. Norvaline was used as the internal standard and 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate was used as the derivatizing agent. The derivative products were separated using an Acquity UPLC™BEH C18 chromatographic column (2.1 mm×150 mm, 1.8 μm), with a flow rate of 0.4 mL/min. The column temperature adopted the programmed temperature rise mode, and the detection wavelength was 260 nm. The quantification of amino acids was performed using an internal standard method. Results The calibration curves of 17 kinds of amino acids showed good linearity with correlation coefficients greater than 0.999. The limits of detection were 0.06-0.14 mg/g, the limits of quantitation were 0.19-0.45 mg/g. Recoveries were in range of 85.1%-108.8%, and the relative standard deviations were within 0.3%-6.7%. The established method was adopted to determine the 3 kinds of animal blood tofu sold online respectively. The 17 kinds of amino acids were found in all products. The content of total amino acids in duck, pig, sheep blood tofu was 63.82, 95.61, 93.52 mg/g. Conclusion The 17 kinds of amino acids can be separated completely within 22 min by use of this method. The established method has good repeatability and high accuracy, and is suitable for the 17 kinds of amino acids determination in animal blood tofu.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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