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Objective To establish a fingerprint spectrum for the volatile compounds of A brand ready-to-eat bird's nest by solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS), and conduct differential analysis on different brands and types of ready-to-eat bird's nest products. Methods Using commercially available ready-to-eat bird's nest as the research object, SPME-GC-MS was used to analyze the volatile substances in 37 batches of A brand ready-to-eat bird's nest samples with different storage periods. The volatile substances were analyzed through the database of NIST 20 and the method of peak area normalization. The “TCM chromatographic fingerprint similarity evaluation system” was used to establish the volatile substances fingerprint spectrum of A brand ready-to-eat bird's nest. Results Under the service of NIST 20 database, 26 kinds of characteristic volatile components were identified in brand A's ready-to-eat bird's nest, mainly including unsaturated compounds such as 5-hydroxymethylfurfural, nonanol, lauryl alcohol, tetrahydro lavender alcohol, benzaldehyde, and so on. A fingerprint spectrum of the volatile substances in this ready-to-eat bird's nest product was established. The evaluation criteria were based on a total of 26 matching peaks, the similarity was greater than or equal to 65.8%, which could be well applied to evaluate the quality of different commercially available ready-to-eat bird's nests. Conclusion By analyzing the composition of volatile substances in ready-to-eat bird's nest and establishing a fingerprint and similarity evaluation method of volatile components for ready-to-eat bird's nest, it is possible to effectively distinguish different quality levels of ready-to-eat bird's nest, providing a scientific basis for quality control and authenticity identification of ready-to-eat bird's nest.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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