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Objective To compare the resistance differences of 18 kinds of bacterial strains and 2 kinds of biological indicators to chlorine-containing disinfectants, hydrogen peroxide and quaternary ammonium compounds. Methods Strains were selected from food, environment and human sources, including gram-negative bacteria, gram-positive bacteria and spore-forming bacteria (6 kinds of strains per category). Species identification was performed using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) and biochemical assays. The 2 kinds of biological indicators, namely Staphylococcus aureus ATCC6538 and Escherichia coli 8099 were used as reference strains. Bacterial suspensions (2.0×108-10.0×108 CFU/mL) were prepared to test their tolerance to sodium hypochlorite (available chlorine 0.00400%-0.00499%), hydrogen peroxide (7.5%), and benzalkonium bromide (3.996 mg/mL). All bacterial strains were treated with disinfectants for 3 minutes. By calculating the killing log value (KL) of disinfectants against different bacterial strains, the differences in resistance of different species of bacterial strains to 3 kinds of disinfectants were compared. Results The bactericidal efficacy ranked as sodium hypochlorite, hydrogen peroxide, benzalkonium bromide. Different species showed significant variations in resistance to disinfectants. Among 3 categories of bacterial strains (gram-negative bacteria, gram-positive bacteria and spore-forming bacteria), there were strains within each category that exhibit higher resistance to disinfectants than 2 kinds of biological indicators. Conclusion Sodium hypochlorite and hydrogen peroxide exhibit superior bactericidal effects compared to benzalkonium bromide. The resistance to disinfectant of bacteria is species-specific and cannot be predicted solely by gram classification. The screening scope for biological indicators should be expanded based on actual needs.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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