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Objective To establish an ultra performance liquid chromatography-photodiode array detector method to analysis 3 kinds of furanocoumarins in dried citrus slices. Methods The samples were ultrasonically extracted with 40 mL ethyl acetate for 40 min twice. Vortex-assisted extraction were then repeated twice using 20 mL of ethyl acetate for 10 min. The ethyl acetate extracts were combined, concentrated to dryness in a rotary evaporator at 30 °C. The determination was carried out using an Acquity UPLC HSS C18 SB column (2.1mm×150 mm, 1.8 μm) with a flow rate of 0.3 mL/min, column temperature of 30 ℃, and detector wavelength of 310 nm. A gradient elution was performed with mobile phase A water and acetonitrile as mobile phase B. Results The 6’,7’-dihydroxybergamottin, epoxybergamottin and bergamottin showed good linearity within 3.00~300.00 μg/mL, 4.32-432.00 μg/mL and 4.12-412.00 μg/mL with correlation coefficients greater than 0.999. The limits of detection were 0.09-0.17 μg/g. The limits of quantitation were 0.31-0.57 μg/g. The average recoveries with 3 concentration levels were 94.4%-99.1%. The relative standard deviations were between 2.7% and 4.7% (n=6). This method could separate 3 kinds of furanocoumarins in dried citrus slices well in 25 minutes. The developed method was successfully applied to determine 3 kinds of furanocoumarins in 18 kinds of dried citrus slices and powders. The content of 6’,7’-dihydroxybergamottin was between 1.52 and 159.04 μg/g. The content of epoxybergamottin was between 49.57 and 60.09 μg/g. The content of bergamottin was between 1.49 and 248.71 μg/g. Conclusion The established method has good repeatability and high accuracy, and can be used for the 3 kinds of furanocoumarins determination in dried citrus slices.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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