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Objective To establish an analytical method for the determination of enrofloxacin residues in complex mixed matrix of the “soil-vegetable” system by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods The sample was subjected to vortex ultrasonic extraction with acidified acetonitrile extract [containing 5% ethylene diamine tetraacetic acid (EDTA)-Mcllvaine buffer solution], settling with centrifugation, getting solvent conversion and being filtered by 0.22 μm nylon filter membrane. The mobile phase was composed of acetonitrile and 0.1% formic acid aqueous solution (containing 2 mmol/L ammonium acetate), and the mixture was subjected to Waters ACQUITY UPLC ® BEH C18 chromatographic column (2.1 mm×100 mm, 1.7 μm) gradient elution separation, using mass spectrometry as a detector for qualitative and quantitative analysis of enrofloxacin. Results In the mass concentration range of 1-200 ng/mL, the standard curve showed a good linear relationship (correlation coefficient=0.9996), the limit of detection of the method was 0.11 μg/kg, the recovery rates ranged from 87.4% to 103.3%, and the relative standard deviations (RSDs) were 0.4% to 4.0% (n=6). Conclusion This method has fast analysis speed, high accuracy and good repeatability, and is suitable for the detection of enrofloxacin residues in complex mixed matrix of the “soil-vegetable” system. It also has the potential to indirectly reveal the risk of antibiotic resistance genes (ARGs) contamination in the system.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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