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Objective To screen lactic acid bacteria from Viili fermented milk and explore the proportion of bacteria in the compound starter cultures. Methods Lactic acid bacteria were isolated using streak plating, and their taxonomic classification was determined through phylogenetic analysis based on 16S rRNA sequences. The composition of strain ratios in mixed cultures was designed with reference to the bacterial abundance observed in Viili fermented milk. Comprehensive analyses were performed to evaluate the acidity, exopolysaccharide production, textural properties and volatile flavor compounds of the mixed fermentation groups. Results Diversity analysis identified 4 bacterial genera, among which Lactococcus, Streptococcus and Leuconostoc were the dominant genera, comprising 49.12%, 40.67% and 10.21% of the total bacterial composition, respectively. Lactococcus lactis H2, Streptococcus thermophilus H17, Leuconostoc mesenteroides H30 and Lactobacillus paracasei H33 were isolated and screened. A total of 20 fermentation trials with different strain compositions were designed to conduct a comprehensive assessment of key parameters, including pH, coagulation time, textural properties, exopolysaccharide content and flavor profile. The results indicated that formulations S13 (H2:H17:H30:H33=11:4:4:1) and S17 (H2:H17:H30:H33=30:35:16:19) demonstrated the highest similarity to Viili fermented milk in terms of physicochemical and sensory properties. Conclusion The blended formulations S13 (H2:H17:H30:H33=11:4:4:1) and S17 (H2:H17:H30:H33=30:35:16:19) exhibit promising potential as compound starter cultures. This study provides a theoretical basis for developing compound starter cultures in Viili fermented milk production.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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