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Objective To investigate false positive interference from spices in hot pot base materials during the detection of thebaine using mass spectrometry, identify interfering matrix components, and optimize analytical methods. Methods The 62 kinds of commonly used spices in hot pot bases were collected. The false positive issues screened by BJS 201802 Determination method of morphine, codeine, papaverine, noscapine and thebaine in food were further analyzed by Fourier transform ion cyclotron resonance mass spectrometry combined with positive pressure electrospray ionization full mass spectrometry scanning. The potential interfering substances were locked through ion fragment analysis and verified. Results The results showed that the detection found interference peaks with retention times similar to those of thebaine in the extracts of bay leaves and pepper, which could lead to false positive results. The molecular weight of the interfering substance was 311.3812 ([M+H]+), with a deviation of less than 2 ppm from the theoretical value of thebaine (311.375), suggesting that it was an isomer. Through the detection of the suspected interfering substance, it was verified that Launobine and Pisatin had no interfering activity. Conclusion The alkaloid substances in spices have significant interference with the detection of thebaine. It is suggested to improve the specificity and accuracy of the detection by optimizing the mass spectrometry conditions, introducing nuclear magnetic resonance technology and perfecting the pretreatment process to avoid false positive misjudgment.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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