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Objective To study the effects of different aquaculture modes on the intestinal microbiota of Opsariichthys bidens. Methods The 16S rRNA gene was amplified by polymerase chain reaction (PCR) using 515F-907R primers using the intestinal microbial DNA of C (monoculture of Opsariichthys bidens), D (mixed culture of Opsariichthys bidens and crayfish), E (mixed culture of Opsariichthys bidens and Macrobrachium nipponense) as templates, and sequenced by Illumina Hiseq2500 sequencing platform. The growth performance of Opsariichthys bidens in different culture modes was simultaneously determined. Results From the perspective of weight and fatness, C>E>D, from the perspective of body length, there was no significant difference between C and E, and there were significant differences between C and D, D and E, which was also C>E>D; from the perspective of hepatosomatic index, there was no significant difference between D and E, but there were significant differences between C and D, C and E, C>E=D; the rule of viscera index and hepatosomatic index was the same, C>D>E; the mixed fish and shrimp farming model significantly increased the diversity and richness of intestinal microbiota in fish compared to the single fish farming model; the mixed farming mode increased the diversity of intestinal microbiota composition in Opsariichthys bidens, and 2 kinds of mixed farming modes had the same effect; the indicator groups for monoculture and 2 kinds of mixed breeding modes of Opsariichthys bidens were different, and the common indicator groups for both mixed breeding modes of Opsariichthys bidens were Microbacteria and Prevotella. Conclusion The comprehensive utilization and quality safety of agricultural products, as well as the single farming mode, result in higher body weight and fat content of Opsariichthys bidens, which is beneficial for increasing the yield of food processing; the mixed farming mode has better microbial diversity and richness, stronger resistance and better quality of Opsariichthys bidens, which is conducive to improving the product quality of food processing. This study can provide theoretical support for the nutritional supply, metabolic homeostasis, epidemic prevention and immunity, food processing, and other aspects of Opsariichthys bidens under different breeding modes.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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