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Objective To establish a new method for simple identification of vitamin A (VA) and unsaturated fatty acids in natural cod liver oil. Methods The cod liver oil sample was saponified and injected into a high-performance liquid chromatography system to detect the content of VA. After saponification of cod liver oil and its excipients, injected them into a gas chromatography system to analyze the localization and percentage of linoleic acid, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), and distinguish fish oil from vegetable oil. Results Under the new method, the linear relationship of VA concentration was good within the range of 30.00-1500.00 IU/mL (r2=0.9999); the average recovery rate was 96.1%. The relative standard deviation of instrument precision was 0.18%, the relative standard deviation of method precision was 1.13%, and the relative standard deviation of intermediate precision was 0.98%. There was a significant difference in the content of fatty acids between vegetable oils, fish oil and cod liver oil. Cod liver oil and fish oil were rich in EPA and DHA, while linoleic acid was low. Corn oil, rapeseed oil and olive oil did not contain EPA and DHA, but they were rich in linoleic acid. Conclusion The detection method establish in this article is simple and accurate, and can be used for the determination of VA and unsaturated fatty acid content in cod liver oil, laying the foundation for the development of ideal quality standards.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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