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Objective To establish a screening and quantitative method for illegally added anthraquinone- containing plant laxatives in weight-loss foods. Methods The anthraquinone glycosides in foods were hydrolyzed into anthraquinone aglycones by the acid hydrolysis extraction method, and 8 kinds of anthraquinone aglycones produced by hydrolysis were determined by ultra performance liquid chromatography-Q-trap-tandem mass spectrometry (UPLC-Q-trap-MS). Multiple reaction monitoring (MRM) was used as a probe for detection, and the enhanced product ion scan (EPI) mode was triggered through information dependent acquisition (IDA). External standard method was adopted for quantification. Results The limit of detection of physcion in weight-loss foods was 5.0 mg/kg, and the limit of quantification was 12.5 mg/kg, with a good linear relationship in the range of 0.25-5.00 μg/mL. The limits of detection of the other 7 kinds of anthraquinone aglycones were 1.0 mg/kg, and the limits of quantification were 2.5 mg/kg, and they had good linear relationships in the range of 0.05-1.00 μg/mL, with correlation coefficients r≥0.992. Spike recovery tests were carried out at three concentration levels of 2.5, 5.0 and 20.0 mg/kg (physcion at 12.5, 25.0 and 100.0 mg/kg), respectively. The average recovery rates of the 8 kinds of anthraquinone aglycones were 80.6%-102.0%, and the relative standard deviations (RSDs) were 2.35%-8.80% (n=6). Conclusion This method has the characteristics of simple operation, high sensitivity and high accuracy, and is suitable for the simultaneous rapid screening and accurate quantification of 8 kinds of anthraquinone aglycones.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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