Objective To establish an efficient method for assessing the authenticity of Citrus reticulata Blanco honey. Methods High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was utilized for the qualitative and quantitative analysis of plant compounds present in Citrus reticulata Blanco honey. The authenticity of commercially available Citrus reticulata Blanco honey was evaluated by integrating characteristic components with HPLC fingerprints. Results A total of 6 kinds of components were identified in Citrus reticulata Blanco honey, namely caffeine, cis,trans-abscisic acid, 5-methoxybrevosin, pinocembrin, kaempferol and chrysin. Caffeine exhibited a relatively high concentration, with an average content of 9.45 mg/kg. Thus, it was regarded as a characteristic component of Citrus reticulata Blanco honey. The authenticity assessment of 6 kinds of Citrus reticulata Blanco honeys available on the market revealed that 4 brands exhibited superior quality, one brand might be adulterated with other components, and one brand could potentially be counterfeit Citrus reticulata Blanco honey. Conclusion This study presents a novel methodology that synergistically combines characteristic component profiling with HPLC-fingerprinting technology for authenticity assessment of Citrus reticulata Blanco honey. This study provides a theoretical foundation for grading Citrus reticulata Blanco honey and enhancing its added value.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |