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Objective To prepare a polydopamine (PDA)-based lateral flow immunochromatography assay for the highly sensitive detection of deoxynivalenol (DON) in cereals. Methods In this paper, PDA was synthesized by dopamine self-oxidative polymerization, and the PDA labeled monoclonal antibody (PDA-mAb) probe was prepared by a one-step conjugation method. Subsequently, the PDA lateral flow immunochromatography assay was established using DON antigen as the test line (T line) and goat anti-mouse antibody as the control line (C line). Results The results showed that the cut-off value of the PDA lateral flow immunochromatography assay for detecting DON was 6.0 ng/mL, the visual limit of detection was 0.9 ng/mL, half maximal inhibitory concentration was 1.13 ng/mL, which were 0.88 times and 3.08 times higher than those of fluorescent microsphere test strip and colloidal gold test strip, respectively. Meanwhile, the PDA lateral flow immunochromatography assay possessed well stability and specificity. In addition, the proposed lateral flow immunochromatography assay has been successfully used for the detection of DON in corn, millet, and oats samples, with minimum detection limits of 8.40, 6.87, and 9.89 μg/kg, recoveries ranging from 80.10%-122.05%, and relative standard deviations less than 12.01%. Conclusion In summary, the PDA lateral flow immunochromatography assay established in this work is sensitive, accurate, rapid and simple, and provides technical support for the detection of mycotoxins in cereals.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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