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Objective To develop a method for analysis of 20 kinds of macrolides and lincomasides in mutton by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods Samples were initially extracted with acetonitrile, and the supernatant was concentrated to near dryness. The residues were further extracted with 50% methanol, and the 2 extracts were combined and diluted with a weak alkaline buffer solution (pH 8.0). The mixture was then concentrated and purified using an HLB solid-phase extraction cartridge. A methanol/5 mmol/L ammonium acetate solution (1:1, V:V) was used for final dilution. Detection was performed in positive ion multiple reaction monitoring mode. A matrix-matched standard curve was prepared for quantification. Chromatographic and mass spectrometry parameters were optimized for the 20 kinds of target compounds, while the effects of different mobile phases, extraction solvents, and purification methods on extraction efficiency and purification quality were thoroughly investigated. Results The 20 kinds of macrolides and lincomasides demonstrated excellent linearity within their respective ranges, with correlation coefficients (r²) exceeding 0.999. Recoveries ranged from 64.7% to 94.2%, the relative standard deviation was between 3.8% and 11.0%. Conclusion The developed method is sensitive, accurate, and suitable for the simultaneous detection of 20 kinds of macrolides and lincomasides in mutton.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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