Objective To establish a simple and efficient method for the determination of 6 kinds of eugenols in infant formula milk powder by gas chromatography-mass spectrometry. Methods After ultrasonic extraction with acetonitrile, the sample was purified and degreased using acetonitrile saturated n-hexane. The n-hexane was discarded, and acetonitrile was extracted and concentrated to a volume of 1 mL. DB-1701 chromatographic column was used to separate without splitting the sample, with an injection volume of 1 μL. Ion monitoring mode for gas chromatography-mass spectrometry determination and external standard method for quantification was used. Results The 6 kinds of eugenols showed a good linear relationship within the range of 20-500 ng/mL, the limit of detection of the 6 kinds of eugenol compounds in this method was 0.007 mg/kg, and the limit of quantitation was 0.02 mg/kg. In 2 matrix samples of milk powder and sheep milk powder, the average recoveries of 6 kinds of eugenols at 3 different concentration levels were between 97.3% and 105.0%, the relative standard deviation (RSD) was between 0.3% and 3.7%. Conclusion The pretreatment operation of this method is simple and fast, and the detection method has high sensitivity and good stability, meeting the requirements of national standards, in order to provide reference basis for establishing national standards or methods for the detection of 6 kinds of eugenols in infant formula milk powder.

gas chromatography-mass spectrometry  /  formula milk powder  /  eugenol