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Objective To establish a method for simultaneous determination the content of 4 kinds of human milk oligosaccharides [2'--fucosyllactose (2'-FL), 3'-sialic acid (3'-SL), 6'-sialic acid (6'-SL) and lactose-N-neotetraose (LNnT)] in milk powder by liquid chromatography-fluorescence method. Methods The samples were dissolved in water, enzymolized with amyloglucosidase or β-galactosidase, derived with 2-aminobenzamide (2-AB) and 2-methylpyridine borane (2-PB), and separated by amide bonding column, detected by fluorescence detector, and quantitated by internal standard methods. Results The 4 kinds of milk oligosaccharides had a good linear relationship in the concentration range of 10-600 μg/mL, and the correlation coefficients (r2) were more than 0.999. The limits of detection and quantification of 4 kinds of milk oligosaccharides were 0.94-2.31 mg/100 g and 3.12-7.69 mg/100 g, respectively; the recovery rates of 4 kinds of milk oligosaccharides were 97.7%-101.5%, and the relative standard deviations (RSD) (n=7) were 0.53%-3.09%. Conclusion This method does not have high requirements for people, equipments and environment for determining the content of 4 kinds of milk oligosaccharides in milk powder. The pre-treatment operation is simple, and it has good accuracy and precision. It can provide a reference for the quality control method of human milk oligosaccharides in milk powder.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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