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Application of adenosine triphosphate bioluminescence method in the microbial detection of chilled meat
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Yan-Zhen ZHANG1, 2, Ning LIU3, Lai-Xue NI3, Wei WANG3, Xian-Qi YAO3, Yun-Guo LIU1, *
Journal of Food Safety & Quality | 2025, 16(6) : 284 - 289
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Journal of Food Safety & Quality | 2025, 16(6): 284-289
• Food Analysis and Detection •
Application of adenosine triphosphate bioluminescence method in the microbial detection of chilled meat
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Yan-Zhen ZHANG1, 2, Ning LIU3, Lai-Xue NI3, Wei WANG3, Xian-Qi YAO3, Yun-Guo LIU1, *
Affiliations
  • 1. College of Life Sciences, Linyi University, Linyi 276005, China
  • 2. College of Engineering, The University of Suwon, Suwon 18323, South Korea
  • 3. Linyi Xincheng Jinluo Meat Products Group Co., Ltd., Linyi 276036, China
Published: 2025-03-25 doi: 10.19812/j.cnki.jfsq11-5956/ts.20241112004
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Objective To investigate the feasibility of using the adenosine triphosphate (ATP) bioluminescence method for detecting the colonies number in chilled meat, and compare its performance with the national standard plate count method. Methods Effects of different concentrations of Triton X-100 and adenosine triphosphate double (Apyrase) on the remove of ATP from the somatic cells of chilled meat were determined. The colonies number in the chilled meat was measured using both the ATP bioluminescence method and the national standard plate count method, and the reproducibility of the ATP bioluminescence method was evaluated. Results The optimal concentrations of Triton X-100 and Apyrase for ATP removal from the somatic cells of chilled meat were found to be 0.2% and 0.10 U/mL, respectively, with the best ATP removal effect observed at these concentrations. The results of the ATP bioluminescence method and the national standard plate count method demonstrated a strong linear relationship, with a correlation coefficient (r²) of 0.9871. The ATP bioluminescence method indicated good reproducibility, and the results were stable and reliable. Conclusion This study further validates the applicability of ATP bioluminescence method for the quick detection of colonies number in chilled meat, demonstrating its advantages over the national standard plate count method.

chilled meat  /  colonies number  /  plate count method  /  adenosine triphosphate bioluminescence method
Yan-Zhen ZHANG, Ning LIU, Lai-Xue NI, Wei WANG, Xian-Qi YAO, Yun-Guo LIU. Application of adenosine triphosphate bioluminescence method in the microbial detection of chilled meat[J]. Journal of Food Safety & Quality, 2025 , 16 (6) : 284 -289 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20241112004
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Year 2025 volume 16 Issue 6
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doi: 10.19812/j.cnki.jfsq11-5956/ts.20241112004
  • Receive Date：2024-11-12
  • Online Date：2025-07-19
  • Published：2025-03-25
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  • Received：2024-11-12
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Affiliations
    1. College of Life Sciences, Linyi University, Linyi 276005, China
    2. College of Engineering, The University of Suwon, Suwon 18323, South Korea
    3. Linyi Xincheng Jinluo Meat Products Group Co., Ltd., Linyi 276036, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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