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Objective To establish a quantitative analysis method for determining the content of 5 kinds of lactose derivatives (3'-galactosyllactose, 4'-galactosyllactose, 6'-galactosyllactose, 3'-sialyllactose, 6'-sialyllactose) in milk and dairy products by pre-column derivatization-high performance liquid chromatography. Methods The proteins in milk and dairy products were precipitated. The 5 kinds of lactose derivatives were reacted with 2-aminobenzamide at 55 ℃ for 120 min to label the fluorescent groups. The 5 kinds of lactose derivatives were separated by high performance liquid chromatography with an amide column and quantified by external standard method. Results The 5 kinds of lactose derivatives exhibited good linear relationships within the concentration range of 0.025-5.000 mg/L, with correlation coefficients (r) all exceeding 0.999. The limits of detection and quantification for the 5 kinds of lactose derivatives ranged from 3.5 to 7.5 mg/kg and 12.0 to 25.0 mg/kg, respectively. The recovery rates for the 5 kinds of lactose derivatives were between 90.8% and 103.3%, with relative deviations in detection results ranging from 2.8% to 5.3% (n=3). Conclusion This method demonstrates good precision, recovery rate, and sensitivity, making it suitable for determining the content of 5 kinds of lactose derivatives in milk and dairy products.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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