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Objective To establish a method for the simultaneous detection of hypoxanthine, guanine, xanthine, and adenine in edible fungi by high performance liquid chromatography, and use this method to the analysis of commercially available edible fungi. Methods The purines (hypoxanthine, guanine, xanthine, and adenine) from edible fungi were extracted by using acid hydrolysis. The chromatographic column was selected based on the resolution and peak shape of the 4 kinds of purines. Detection conditions, including the mobile phase ratio, flow rate, column temperature, and injection volume, were optimized, and the methodology was validated. Results The results showed that using Insert Sustain AQ-C18 column with a mobile phase consisting of 50 mmol/L ammonium acetate solution (pH 4.0) and methanol (V:V=97:3), at a flow rate of 0.6 mL/min and the column temperature of 30 ℃, with an injection volume of 10 μL and detected at 254 nm, the 4 kinds of purines could be completely separated with good peak shapes. And the methodological validation results showed that this method exhibited a good linear relationship within the detected range. Conclusion A high performance liquid chromatography method is established through the optimization of detection conditions, which effectively differentiates and simultaneously detects the 4 kinds of purines in edible fungi. The content of hypoxanthine and xanthine (uric acid precursor substances) are very low or undetectable in the detected edible fungi. Based on the purine intake guidelines in the Encyclopedia of Chinese nutritional science, the dietary intake of the currently available commercial edible mushrooms is determined.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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