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Objective To establish a method for the determination of chlorophyll a and chlorophyll b content in vegetables by convergence chromatography (CC). Methods The Soxhlet extraction method was used for efficient extraction of chlorophyll from vegetables and their products, followed by separation and detection using an ultra-performance convergence chromatography system coupled with a ultraviolet-visible detector. Results Through comparison of the stability of different solvents, petroleum ether was selected as the extraction solvent, and the extraction conditions were optimized. The method was validated with a limit of detection of 0.02 mg/kg, a limit of quantification of 0.06 mg/kg, a linear range of 0.06-800.00 mg/kg, a spike recovery rate of 99%-102%, and a relative standard deviation of 2.2%-14.0% for real samples. The reliability and accuracy of the method were confirmed through precision testing of real samples, including kale, fresh mustard greens, pickled mustard greens, dried pickled mustard greens, steamed dried pickled mustard greens, and boiled dried pickled mustard greens, as well as cross-validation with existing standard methods. Conclusion This study establishes a CC-based method for detecting chlorophyll a and chlorophyll b in vegetables, demonstrating high sensitivity, a wide linear range, good specificity, and precision. The method is not only suitable for laboratory research but also has practical application potential.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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