Objective To establish a rapid colloidal gold card method for the determination of fumonisin and T-2/HT-2 toxin in Triticum aestivum L. and Zea mays. Methods The limit of detection, limit of quantitative, precision and accuracy of the method were evaluated in 6 laboratories. Results The limits of detection of fumonisin and T-2/HT-2 toxin methods were 150 μg/kg and 5 μg/kg, respectively. The limits of quantitation were 450 μg/kg and 11 μg/kg, respectively. When the fumonin content in Triticum aestivum L. and corn ranged from 426.39 to 3897.22 μg/kg, the relationship between the method repeatability standard deviation S_r and reproducibility standard deviation S_R and fumonin content was obtained, namely, repeatability standard deviation S_r=1.478m^0.600 and reproducibility standard deviation S_R=4.269m^0.591. When the T-2/HT-2 toxin content in Triticum aestivum L. and Zea mays ranged from 426.39 to 3897.22 μg/kg, the relation between repeatability standard deviation S_r and reproducibility standard deviation S_R and T-2/HT-2 toxin content m was as follows: Repeatability standard deviation S_r=3.1901m^0.218, reproducibility standard deviation S_R=0.2507m^0.492 (Triticum aestivum L. matrix). Repeatability standard deviation S_r=0.8357m^0.333, reproducibility standard deviation S_R=1.1231m^0.585 (Zea mays matrix). The recoveries of fumonisin and T-2/HT-2 toxin ranged from 95.42% to 101.90% and 83.30% to 118.20% respectively. There was no significant difference between the paired t test and the standard method. Conclusion The method has good accuracy and can be widely used for rapid and quantitative determination of fumonisin and T-2/HT-2 toxin in Triticum aestivum L. and Zea mays.

colloidal gold  /  method joint verification  /  Triticum aestivum L.  /  Zea mays