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Objective To explore a method that can monitor the immunoaffinity column capacity of zearalenone (ZEN) in real time during detection. Methods The 2 kinds of artificial structural analogues of ZEN (α-ZEL-G and β-ZEL-G) were synthesized by esterification of α-zearalanol (α-ZEL) and β-zearalanol (β-ZEL) with glutaric anhydride in this study. The products were purified and identified by fluorescence, ultraviolet and high-resolution mass spectrometry. A competitive enzyme-linked immunosorbent assay (ELISA) curve was established using the same antibody with immunoaffinity columns, and their affinity with the antibody was compared by half maximal inhibitory concentration (IC50). α-ZEL-G and β-ZEL-G were used as column capacity tracers and added to the immunoaffinity column together with different concentrations of ZEN to explore a method for real-time monitoring of immunoaffinity column capacity. Results The IC50 of the competitive ELISA curves of ZEN, α-ZEL-G and β-ZEL-G were 2.0, 1.3 and 10.0 ng/mL, respectively, indicating that the affinity of α-ZEL-G with antibody was slightly higher than that of ZEN, while the affinity of β-ZEL-G with antibody was significantly lower than that of ZEN with antibody. The experimental results showed that: α-ZEL-G in immunoaffinity columns with different column sizes would affect the recovery rates of ZEN. Whereas the recovery rates of ZEN with different concentration were more than 80% while the adding concentrations of β-ZEL-G was 50% of the column capacity. Conclusion β-ZEL-G with lower antibody affinity is more suitable for real-time monitoring of column volume. It shows good application results in the test of actual samples.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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