Objective To detect aflatoxin B1 (AFB1) in peanut oil by laser induced fluorescence and explore the effects of temperature on the detection results. Methods The spectral information of AFB1 contaminated peanut oil at temperatures of 10, 20, 30, 40 and 50 ℃ was collected, linear discriminant analysis (LDA) model was established for qualitative analysis, and partial least squares regression model was established for quantitative analysis. Results The accuracy of the single-temperature LDA detection model in predicting samples at this single temperature exceeded 84%. The global LDA model was lower prediction accuracy than the single-temperature LDA model. The partial least squares regression (PLSR) model could not achieve quantitative prediction at either single temperature or mixed temperature. The PLSR quantitative model developed for single-variety peanut oil demonstrated optimal stability at 20 °C, yielding the most accurate sample predictions. Conclusion This study proposes that a global model can be established during qualitative discriminant analysis to adapt to the impact of temperature on the detection process. In quantitative analysis, the single temperature model can achieve more accurate predictions than the global model.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |