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Tetracycline antibiotics are widely used in animal husbandry, and their main ways of entering milk are through environmental exposure and direct ingestion by cows. Tetracyclines accumulate toxicity through the food chain and pose a threat to human health. Molecular imprinting technology can customize high molecular network polymers that specifically bind to tetracyclines, with many advantages such as high selectivity, good mechanical properties, low cost, high temperature and acid-base resistance, and reusability. It plays an important role in the detection of tetracycline residues in milk. The combination of molecular imprinting and solid-phase extraction technology can simplify the pretreatment process, optimize methods, and effectively solve the separation and purification problems caused by the complexity of milk matrix, including four solid-phase extraction and chromatography techniques: Solid-phase extraction, solid-phase microextraction, dispersed solid-phase extraction, magnetic solid-phase extraction, etc. The specific recognition ability based on molecular imprinting technology combined with sensors (including 5 types of sensors: Molecular imprinting electrochemistry, fluorescence, photonic crystal, plasma resonance, and photoelectric sensors) can improve selectivity, stability, and response speed. The operation is simple, especially suitable for rapid screening on site. This article introduced the research progress of the above-mentioned technologies and sensors in detecting tetracycline residues in milk, analyzed and discusses their application effects, and pointed out the future direction of molecular imprinting technology in this field, providing reference for subsequent research and application.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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