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Objective To compare the changes in muscle nutritional components and quality indicators of Trionyx sinensis at different stages of ecological purification breeding. Methods Nutritional parameters such as morphological index and protein, fat, amino acid and fatty acid in muscle and skirt were measured during the 0th, 15th and 30th d of purification, and the nutritional and quality changes of Trionyx sinensis were compared with the textural and structural characteristics. Results With the extension of ecological purification breeding time, the crude fat of Trionyx sinensis showed a trend of rapid decline followed by a slight increase, while the crude protein showed a trend of initial increase followed by a decrease. The total amino acid content showed a decreasing trend. In the entire ecological purification breeding process, there were significant changesin the crude protein and crude fat content in the muscles of Trionyx sinensis (P<0.05), as well as in the moisture and ash content of their skirts. There were significant differences in the content of saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids before and after purification (P<0.05), and the content of saturated fatty acids and polyunsaturated fatty acids increased at 15 days. Except for valine and proline, all other amino acids decreased, and there was a significant decrease in cysteine, lysine, glutamate, histidine, aspartic acid, glycine, and alanine in the purified muscles after 30 days compared to the untreated ones (P<0.05). In the whole texture mode, the hardness, adhesion and mastication of the muscle of Trionyx sinensis purified for 15 days were significantly improved (P<0.05) compared with that of the muscle before purification. Conclusion Compared with traditional pond aquaculture of Trionyx sinensis, the crude protein content, essential amino acids, and total umami amino acids are significantly increased, and the optimal purification time is 15 d. Ecological purification breeding can improve the quality of Trionyx sinensis and better meet the needs of consumers. It is a purification breeding method worth applying in production practice, which is conducive to increasing the supply of high-quality and cost-effective Trionyx sinensis and providing research data for improving the quality of high-quality aquatic products.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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