Home Journals Articles Updates About
CN
image
收藏切换
Establishment of a rapid detection technology system for Neofusicoccum laricinum based on CEEA isothermal amplification
收藏切换
PDF
Zhengqiang Liu1, Tingqian Pei1, Wenjing An3, Lanze Liu1, Yeting Zhu1, Yingmei Liang2, *
Tree Health | 2026, 3(2) : 40 - 48
Less
收藏切换
Tree Health | 2026, 3(2): 40-48
• Research paper •
Establishment of a rapid detection technology system for Neofusicoccum laricinum based on CEEA isothermal amplification
Full
Zhengqiang Liu1, Tingqian Pei1, Wenjing An3, Lanze Liu1, Yeting Zhu1, Yingmei Liang2, *
Affiliations
  • Key Laboratory of Forest Cultivation and Protection，Ministry of Education，Beijing Forestry University，Beijing 100083，China
  • Beijing Forestry University Museum，Beijing 100083，China
  • Beijing Jingxi Forest Farm Management Office，Beijing 102300，China
Published: 2026-04-25 doi: 10.27035/j.cnki.issn2097-5279.20260205
Outline
收藏切换

Neofusicoccum laricinum (Sawada) Y. Hattori & C. Nakash is a quarantine pest of imported plants and a forestry quarantine pest in China. It is also one of only two fungal plant pathogens listed in the Catalogue of Key Managed Invasive Alien Species. Developing rapid, accurate, and visual detection method is crucial for effective prevention and control of this disease. In this study, a method combining composite enzyme index amplification （CEEA） technique with lateral flow dipstick （LFD） technology was developed. Using specifically designed CEEA primer sets and probes for N. laricinum, followed by optimization of reaction conditions （39 ℃ for 15 minutes）, the method could specifically detect N. laricinum strains from diverse regions with a limit of detection (LOD) as low as 100.0 fg·µL−1, the entire process to be completed in 30 minutes. This technology requires no expensive instruments and does not identify other fungi on Larch, demonstrating good applicability. With its high efficiency, speed, and sensitivity, this technology provides strong support for in-situ rapid detection and identification of pathogens in the forest, as well as for the early warning of disease.

Neofusicoccum laricinum  /  Larch  /  composite enzyme index amplification  /  lateral flow test strips  /  detection technology
Zhengqiang Liu, Tingqian Pei, Wenjing An, Lanze Liu, Yeting Zhu, Yingmei Liang. Establishment of a rapid detection technology system for Neofusicoccum laricinum based on CEEA isothermal amplification[J]. Tree Health, 2026 , 3 (2) : 40 -48 . DOI: 10.27035/j.cnki.issn2097-5279.20260205
Appendix
Less
Year 2026 volume 3 Issue 2
PDF
11
2
Cite this Article
BibTeX
Article Info
doi: 10.27035/j.cnki.issn2097-5279.20260205
  • Receive Date：2025-10-24
  • Online Date：2026-07-30
  • Published：2026-04-25
Article Data
Affiliations
History
  • Received：2025-10-24
  • Revised：2025-12-22
Affiliations
    Key Laboratory of Forest Cultivation and Protection，Ministry of Education，Beijing Forestry University，Beijing 100083，China
    Beijing Forestry University Museum，Beijing 100083，China
    Beijing Jingxi Forest Farm Management Office，Beijing 102300，China
References
Share
https://castjournals.cast.org.cn/joweb/smyx/EN/10.27035/j.cnki.issn2097-5279.20260205
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House