Biometallurgy has drawn the attention of the academic community in recent years as an effective way to achieve in-situ resource utilization (ISRU) in future interstellar bases. However, the mechanism of interaction between Streptomyces and minerals is still unclear under the effect of microgravity. To investigate this issue, simulating the leaching of elements from minerals by Streptomyces sp. R76 under microgravity using clinostats was studied. The results show that the simulated microgravity environment significantly accelerated Streptomyces sp. R76's development, differentiation, and metabolic capacity, which is primarily manifested in the early development and differentiation of aerial hyphae and spores, as well as the increase in organic acid. Streptomyces sp. R76 can produce lactic acid and citric acid at concentrations of 8.6 and 0.17 mmol/L under simulated microgravity and 5.8 and 0.04 mmol/L under gravity, respectively. When Streptomyces sp. R76 is co-cultured with minerals, the leaching rate of rare earth elements under simulated microgravity is 3‰, the leaching rate of calcium is 0.11‰.The leaching rate of rare earth elements under gravity is only 0.33‰ and the leaching rate of calcium is 0.08‰.The lactic acid and citric acid produced by Streptomyces sp. R76 under microgravity conditions are important factors in promoting the leaching of calcium, silicon, and rare earth elements from minerals.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |