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Drought is one of the most serious abiotic stresses to land plants. Environmental water deficiency triggers an osmotic stress signaling cascade. OSCA1 was described as a hyperosmolarity-gated calcium-permeable channel and its function in osmosensing in plants, homologues of OSCA genes are found throughout eukaryotes. Water is crucial to plant growth and development. As one of the primary horticulture commodities, tomato plays an important role in vegetable research. To enhance the survival and yield of tomatoes in drought conditions, it is essential to thoroughly study the function of SlOSCA genes and their drought-resistant mechanisms. Based on reverse genetics, we selected SlOSCA1.1/1.2/1.6/2.3/3.1 as our target genes through bioinformatics analysis, the slosca mutant of tomato was made by CRISPR/cas9-mediated gene editing directly in AC (Ailsa Craig). To explore the role of tomato SlOSCA protein family members in the response to osmotic stress. Here, we found impaired guard cell osmotic stress signaling and attenuated plant responses to osmotic stress in slosca3.1/1.1 plants, impaired osmotic stress signaling and attenuated plant responses to osmotic stress in slosca1.6/1.2/2.3 plants, the slosca1.6/1.2/2.3 plants showed lower water use efficiency and poorer drought resistance, there is genetic redundancy in OSCA. In this study, slosca mutants were constructed and then the physiological phenotypes of the mutants were investigated, which revealed that multiple genes of the SlOSCA family cooperatively regulate responses to osmotic stress, this study provides a reference for subsequent analysis of the biological functions of OSCA gene family under different stress conditions and different physiological processes.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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