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Establishment and Optimization of Tissue Culture and Rapid Propagation Technology System of Reke Xiamo
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Xiaoyun YU1, 2, 3, Huanlin YU1, 2, 3, Shuangshuang YI1, 2, 3, Yi LIAO1, 2, 3, Shunjiao LU1, 2, 3, Xiaoyan LUO1, 2, 3, *
Chinese Journal of Tropical Crops | 2025, 46(11) : 2603 - 2608
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Chinese Journal of Tropical Crops | 2025, 46(11): 2603-2608
• Germplasm Resources, Genetics & Breeding •
Establishment and Optimization of Tissue Culture and Rapid Propagation Technology System of Reke Xiamo
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Xiaoyun YU1, 2, 3, Huanlin YU1, 2, 3, Shuangshuang YI1, 2, 3, Yi LIAO1, 2, 3, Shunjiao LU1, 2, 3, Xiaoyan LUO1, 2, 3, *
Affiliations
  • 1.Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Innovation in South China, Ministry of Agriculture and Rural Affairs, Haikou, Hainan 571101, China
  • 2.Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Haikou, Hainan 571101, China
  • 3.Engineering and Technology Research Center for Innovation and Utilization of Tropical Ornamental Plant Germplasm, Danzhou, Hainan 571737, China
Published: 2025-11-25 doi: 10.3969/j.issn.1000-2561.2025.11.006
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It aimed to establish and optimize an efficient tissue culture rapid propagation system for Dendrobium Reke Xiamo to address the inefficiency and high cost associated with its traditional propagation methods. By systematically screening the concentration ratios of cytokinin (6-BA) and auxin (NAA), the addition of potato powder, and rooting medium formulations, an efficient and stable tissue culture rapid propagation system was successfully developed. The experimental results showed that the combination of 6-BA at 3 mg/L and NAA at 0.5 mg/L in MS medium was the optimal shoot induction medium, with the highest shoot induction rate achieved when 80 g/L of potato powder was added. The proliferation medium consisting of 6-BA at 2 mg/L and NAA at 0.5 mg/L resulted in an average proliferation coefficient of 5.63. During the rooting stage, the combination of NAA at 0.5 mg/L and IBA at 0 mg/L yielded the most significant results. By establishing a stage-specific precise hormonal regulation system (induction-proliferation-rooting), the propagation bottleneck was successfully overcome, and the proliferation coefficient increased by over 30%. This technical system would provide reliable technical support for the scaled production and commercialization of Dendrobium Reke Xiamo.

Reke Xiamo  /  dendrobium  /  explant  /  tissue culture rapid propagation
Xiaoyun YU, Huanlin YU, Shuangshuang YI, Yi LIAO, Shunjiao LU, Xiaoyan LUO. Establishment and Optimization of Tissue Culture and Rapid Propagation Technology System of Reke Xiamo[J]. Chinese Journal of Tropical Crops, 2025 , 46 (11) : 2603 -2608 . DOI: 10.3969/j.issn.1000-2561.2025.11.006
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Year 2025 volume 46 Issue 11
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Article Info
doi: 10.3969/j.issn.1000-2561.2025.11.006
  • Receive Date：2025-06-12
  • Online Date：2026-06-24
  • Published：2025-11-25
Article Data
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History
  • Received：2025-06-12
  • Accepted：2025-07-28
Funding
Affiliations
    1.Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Innovation in South China, Ministry of Agriculture and Rural Affairs, Haikou, Hainan 571101, China
    2.Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Haikou, Hainan 571101, China
    3.Engineering and Technology Research Center for Innovation and Utilization of Tropical Ornamental Plant Germplasm, Danzhou, Hainan 571737, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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