NF-YB transcription factors play key regulatory roles in plant growth, development, and stress responses. In this study, HMMER and BLAST were used to identify members of the wheat NF-YB gene family, and their physicochemical properties, phylogenetic relationships, and gene structures were analyzed. Using wheat varieties including Lantian 134, Longzimai 1, and Chang 6878, the expression pattern of TaNF-YB-2 at the seedling stage under drought and salt stress was examined. A total of 10 TaNF-YB family members were identified in the wheat genome and sequentially named TaNF-YB-1 to TaNF-YB-10. These proteins were unstable and acidic. The TaNF-YB members in common wheat showed high similarity to their homologs in spelt wheat, indicating evolutionary conservation. TaNF-YBs genes were mainly located on homologous groups 1 and 4, with five-gene pairs showing collinearity. Cis-element analysis suggested that TaNF-YB gene family are involved in stress responses. Under drought and salt stress, the expression of TaNF-YB-2 increased in both roots and leaves. Under drought stress, the relative expression levels of TaNF-YB-2 in three drought-resistant varieties (Lantian 134, Longzimai 1, and Chang 6878) were consistently significantly higher than in two drought-sensitive varieties (Xikemai 518 and Lanhangxuan 121). Under salt stress, the relative expression levels of TaNF-YB-2 in the three salt-tolerant varieties were significantly higher than those in the salt-sensitive materials—in leaves after 4 h and in roots after 6 h of treatment.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |