Under the background of the “Two-Wings Theory”, it is of great significance to further strengthen the coordination and cooperation among the main bodies of science popularization policy-making in China for improving the effectiveness of science popularization. Taking 648 subjects of science popularization policy making at the national level in China from 1994 to 2020 as the research object, this paper quantified the colletive evolution of issues of science popularization policymaking in China from three dimensions of policy subjects, policy types and policy intensity, and found that: The scale of cooperation of the main body in the formulation of science popularization policy in China shows an evolution trend from “preliminary development” to “rapid development” to “steady development”; The main body cooperative network shows the evolution characteristic from “dispersion-coupling type” to “center-edge type” and then to “loose type”; The scope and depth of cooperation network among major entities are expanding; The supporting system to promote the cooperation and coordination of the main body of science popularization policy-making in China needs to be further strengthened and improved. Finally, based on the analysis of characteristics, several policy suggestions are put forward to enhance the coordination between different government departments and people’s organizations in making science popularization policy in China which could enhance the effectiveness of science popularization policy.